Applications of Molecular Diagnostics for Quality Control in Rearing of Spodoptera frugiperda (Lepidoptera: Noctuidae) Larvae for Experimental Use

نویسندگان

چکیده

Nuestros resultados demuestran que a través de un diagnóstico molecular en Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), usando secuenciación del 16S rDNA por Illumina MiSeq, larvas saludables y muertas mostraron una abundancia relativa alta Enterococcus (Lactobacillales: Enterococcaceae) con promedio 89.2% and 94.8% respectivamente. Mientras Pseudomonas (Pseudomonadales: Pseudomonadaceae) Acetobacter (Rhodospirillales: Acetobacteraceae) presentaron menor relativa. Adicionalmente, metagenomica secuencias virus relacionados Alphabaculovirus muy comparada las saludables. Se detectaron virales relacionadas al Encrespamiento amarillo la hoja tomate, Heliothis zea (Boddie) Noctuidae) nudivirus, Clostera anachoreta (Denis & Schiffermüller) Notodontidae) granulovirus Tipula oleracea L. (Diptera: Tipulidae) nudivirus. detección agentes patogénicos es primordial durante crianza insectos como control calidad para evitar su dispersión o adquisición.

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ژورنال

عنوان ژورنال: Florida Entomologist

سال: 2023

ISSN: ['1938-5102', '0015-4040']

DOI: https://doi.org/10.1653/024.106.0212